FastQCFastQC Report
Fri 10 Feb 2017
ERR1147806.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147806.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2049961
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99840.48703365576223157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57780.2818590207325895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48250.23537033143557368No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76850.052.6874731
GGTATCA41300.049.0197681
AACGCAG97550.040.7379726
TCAACGC98050.040.6515774
CAACGCA99700.039.8594635
ATCAACG100600.039.6802833
TATCAAC104350.038.6543162
ACGCAGA115950.034.169857
CGCAGAG117500.033.714998
GCAGAGT129250.030.696019
GTACATG112350.029.8915481
TACATGG115900.028.7965342
ACATGGG118050.027.7674393
AGAGTAC121950.026.33895910-11
GAGTACT57800.026.2935812-13
CATGGGG55300.024.743034
AGTACTT62000.024.36849212-13
CAGAGTA127900.023.6021810-11
GTACTTT65950.023.17954814-15
CATGGGT23100.022.148114
Produced by FastQC (version 0.11.5)