FastQCFastQC Report
Fri 10 Feb 2017
ERR1147805.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147805.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2502304
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106150.4242090489404964No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.24893058557233652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.19214292108392905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83450.055.2929271
GGTATCA45100.053.5973971
TCAACGC108400.041.8188974
AACGCAG109400.041.5453956
ATCAACG110200.041.189813
CAACGCA111500.040.7629245
TATCAAC117800.038.784912
ACGCAGA126800.035.889857
CGCAGAG130900.034.8049138
GCAGAGT144750.031.5568799
GTACATG129350.031.0232771
TACATGG133050.029.9128722
ACATGGG133500.029.0099263
CATGGGG66800.027.7859334
AGAGTAC136900.026.56658410-11
CAGAGTA141850.024.71708110-11
CATGGGT30300.024.5422444
GAGTACT65500.023.24563212-13
AGTACTT69400.023.0534312-13
ATGGGGG35100.021.6945045
Produced by FastQC (version 0.11.5)