FastQCFastQC Report
Fri 10 Feb 2017
ERR1147802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4736332
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122420.2584700565754259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69950.14768812659247704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57270.12091635468121743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA143300.049.0170751
AACGCAG172450.040.3896836
TCAACGC174600.039.653864
CAACGCA177050.039.273115
GGTATCA79600.039.2606771
ATCAACG182450.037.8825343
ACGCAGA196500.035.4160467
TATCAAC199700.034.938262
CGCAGAG199400.034.9300588
GTACATG215150.032.121991
ACATGGG215000.031.3726583
GCAGAGT223300.031.3512829
CATGGGG102200.030.67164
TACATGG233250.029.6323282
ATGGGGG56550.027.6630385
AGAGTAC214250.026.35921910-11
GAGTACT99600.024.03615212-13
CATGGGT45200.022.6342854
CAGAGTA223750.022.5818110-11
CATGGGA99000.021.9297894
Produced by FastQC (version 0.11.5)