FastQCFastQC Report
Fri 10 Feb 2017
ERR1147801.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147801.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6123593
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124280.20295274359350796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70110.11449160648005181No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144450.048.8805051
AACGCAG156950.044.191666
TCAACGC164000.042.4370464
CAACGCA166550.041.7515835
ATCAACG173000.040.2293363
ACGCAGA179550.038.596127
CGCAGAG185150.037.5217638
GGTATCA77950.037.500231
TATCAAC196150.035.936872
GCAGAGT216450.032.2607579
GTACATG207500.031.8186681
ACATGGG206900.030.992843
TACATGG225000.029.2139282
CATGGGG102450.027.5793934
AGAGTAC201850.027.2562710-11
CATGGGT56100.023.6453154
CAGAGTA217200.022.79700910-11
GAGTACT94750.022.19032512-13
ATGGGGG45850.021.017395
AGTACAT170550.019.79099312-13
Produced by FastQC (version 0.11.5)