FastQCFastQC Report
Fri 10 Feb 2017
ERR1147800.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147800.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4241441
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128780.3036232261629951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67000.1579651821161723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60900.14358327747574468No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120700.048.432721
AACGCAG145650.039.4909136
TCAACGC150750.038.075994
GGTATCA67900.038.049991
CAACGCA152650.037.524135
ATCAACG155350.036.9102483
ACGCAGA169050.033.9541857
CGCAGAG173350.033.1450848
TATCAAC173600.033.1335642
GTACATG184500.032.652671
ACATGGG184450.031.7642613
TACATGG191850.031.31492
GCAGAGT192950.030.1172719
CATGGGG99150.029.2157754
AGAGTAC183300.026.98115310-11
ATGGGGG52000.024.7076595
CATGGGT45400.024.500054
GAGTACT88000.023.62246712-13
AGTACTT103750.023.13215812-13
ATGGGTA27000.022.2504525
Produced by FastQC (version 0.11.5)