FastQCFastQC Report
Fri 10 Feb 2017
ERR1147799.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147799.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3084770
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101050.3275770965096263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.1698667972004396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49640.16091961475247749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88800.053.8242451
AACGCAG107250.044.201156
TCAACGC108000.043.5086754
CAACGCA108600.043.377845
GGTATCA47000.042.045121
ATCAACG112950.041.707243
TATCAAC123200.038.5269552
ACGCAGA125850.037.4315227
CGCAGAG130250.036.2085958
GTACATG142000.032.485441
GCAGAGT146950.032.2151159
ACATGGG141900.031.3539123
TACATGG148100.031.1257022
CATGGGG77200.027.9680294
AGAGTAC137200.026.65816110-11
CAGAGTA141800.023.80119710-11
GAGTACT65150.023.50563612-13
ATGGGGG37350.023.2506335
AGTACTT70300.022.84113112-13
GTACTTT73500.020.87572314-15
Produced by FastQC (version 0.11.5)