FastQCFastQC Report
Fri 10 Feb 2017
ERR1147798.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147798.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228542
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59770.26820225959394084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34750.15593154627554698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28980.13004017873569357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23350.062.2158741
GTATCAA43100.055.9468961
TCAACGC56550.041.9699864
CAACGCA58450.040.5039255
ATCAACG59300.039.8230323
AACGCAG60250.039.4913066
TATCAAC71000.034.0139052
ACGCAGA71750.032.9958767
GTACATG81750.031.6082341
CGCAGAG76250.031.1251958
TACATGG84800.030.372522
ACATGGG85100.029.7768363
GCAGAGT85750.027.746299
CATGGGG46450.025.355844
AGAGTAC76900.025.02172710-11
CAGAGTA81850.023.32682610-11
GAGTACT36700.023.01397112-13
AGTACTT39800.020.84780912-13
GTCTTAG7250.019.7093121
CATGGGT24000.019.5800764
Produced by FastQC (version 0.11.5)