FastQCFastQC Report
Fri 10 Feb 2017
ERR1147797.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147797.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3196765
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133930.41895478710508904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74310.2324537462090582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61620.19275736564933613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95550.057.1865271
GGTATCA48350.053.305641
TCAACGC126100.042.6870234
AACGCAG128050.042.3156626
CAACGCA129050.041.8034025
ATCAACG129600.041.58013
TATCAAC141200.038.6287422
ACGCAGA151100.035.821137
CGCAGAG156550.034.6853528
GTACATG157250.032.552891
GCAGAGT171250.031.812179
TACATGG164700.030.7696062
ACATGGG161950.030.666763
CATGGGG87500.028.2099554
AGAGTAC162150.026.97702810-11
GAGTACT76450.024.46648412-13
CATGGGT37750.024.2642084
ATGGGGG45850.023.9990885
CAGAGTA169050.023.88817610-11
AGTACTT82150.023.31184812-13
Produced by FastQC (version 0.11.5)