FastQCFastQC Report
Fri 10 Feb 2017
ERR1147796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5895137
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212320.3601612651241184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116180.1970776930205354No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94610.16048821257249832No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68450.063.5773431
GTATCAA146650.063.125781
TCAACGC192950.047.3525854
CAACGCA197800.046.161445
ATCAACG202050.045.3082243
AACGCAG206250.044.3279046
TATCAAC219550.041.9955142
ACGCAGA242550.037.6692737
CGCAGAG251650.036.3046348
GTACATG250350.032.5071831
GCAGAGT283600.032.4243359
TACATGG262550.030.8807962
ACATGGG261850.030.508493
CATGGGG162100.028.5124994
AGAGTAC260050.027.9612210-11
CAGAGTA273150.024.81288310-11
GAGTACT129050.024.79638512-13
AGTACTT143950.024.06846412-13
ATGGGGG83250.024.0077955
GTACTTT152000.021.22854614-15
Produced by FastQC (version 0.11.5)