FastQCFastQC Report
Fri 10 Feb 2017
ERR1147795.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147795.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4966483
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147810.29761503260959515No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78690.15844210077835763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66610.1341190536643335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132600.053.2848851
GGTATCA68550.044.4589651
AACGCAG159650.043.625826
TCAACGC160000.043.5303884
CAACGCA163800.042.484225
ATCAACG168050.041.4451833
TATCAAC184150.038.3400042
ACGCAGA185550.037.5042577
CGCAGAG189700.036.7132958
GTACATG205250.034.5402451
GCAGAGT216500.032.3609439
ACATGGG211100.032.288813
TACATGG225200.031.060762
CATGGGG102500.029.071644
AGAGTAC204050.027.38354110-11
CATGGGT47300.025.149224
ATGGGGG51550.023.9988445
GAGTACT98500.023.60858512-13
CAGAGTA215100.023.32244710-11
CATGGGA92400.021.8858094
Produced by FastQC (version 0.11.5)