FastQCFastQC Report
Fri 10 Feb 2017
ERR1147794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555349
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82620.32332178500862313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43530.17034855121550913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.149842545969259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79350.052.4440161
GGTATCA43150.042.770721
AACGCAG95650.042.53666
TCAACGC98950.041.4185644
ATCAACG100100.041.0021483
CAACGCA100500.040.602215
TATCAAC110150.037.423882
ACGCAGA109700.036.9252627
CGCAGAG112800.035.8043068
GTACATG128450.034.5756571
TACATGG130800.033.6530232
ACATGGG127600.033.563983
CATGGGG65400.033.0156974
GCAGAGT125750.032.1644069
ATGGGGG34700.030.6842275
AGAGTAC122900.026.27931610-11
GAGTACT53250.025.3555112-13
CATGGGT23450.024.8584964
TGGGGGG28700.023.6273276
CATGGGA57150.023.5225184
Produced by FastQC (version 0.11.5)