FastQCFastQC Report
Fri 10 Feb 2017
ERR1147793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755036
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145880.3884916149938376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79130.21073033654004916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71930.19155608627986523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114500.053.035531
GGTATCA61850.047.262221
AACGCAG141600.042.6773766
TCAACGC142800.042.2354354
CAACGCA145250.041.523035
ATCAACG147250.040.9994473
TATCAAC157850.038.6240772
ACGCAGA161650.037.4197547
CGCAGAG166700.036.35518
GTACATG165500.032.6633071
GCAGAGT188800.032.036559
ACATGGG165900.031.227633
TACATGG172700.030.894342
CATGGGG78450.027.7495164
AGAGTAC173500.027.47456410-11
GAGTACT88750.025.90048612-13
CAGAGTA184050.024.36476910-11
AGTACTT101700.023.77203612-13
CATGGGA73250.022.9797924
CATGGGT37550.022.492914
Produced by FastQC (version 0.11.5)