FastQCFastQC Report
Fri 10 Feb 2017
ERR1147792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1400325
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95430.6814846553478656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55980.39976434042097375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50470.3604163319229465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15260.10897470230125149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95350.038.895451
AACGCAG108350.033.6564456
GGTATCA64600.033.5428771
TCAACGC117300.031.1898844
ATCAACG117550.031.1741583
TATCAAC118100.031.1308352
CAACGCA117900.031.0816145
ACGCAGA122000.029.8409567
ACATGGG104700.029.4888173
CGCAGAG123800.029.4049828
TAACGCA1454.073585E-628.7188284
TATCACG1254.888329E-528.555742
GTACATG114300.028.124121
TATAACG1306.38573E-527.4574412
TACATGG115300.027.3979072
GCAGAGT135350.027.0715169
CATGGGG49600.025.9065024
CATGGGT17400.025.2999214
GAGTACT65400.025.05728512-13
ATGGGGG21750.024.889655
Produced by FastQC (version 0.11.5)