FastQCFastQC Report
Fri 10 Feb 2017
ERR1147791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771851
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133120.35293016611737843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71980.19083468567554762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64290.17044681775605666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95450.058.6234471
GGTATCA48750.057.8792841
TCAACGC127500.043.0608524
CAACGCA129750.042.3141335
ATCAACG130550.042.009273
AACGCAG135750.040.5753486
TATCAAC146450.037.855512
ACGCAGA158550.034.589967
CGCAGAG165450.033.1815958
GTACATG171400.032.4381031
ACATGGG172650.031.2486883
TACATGG177900.031.0963632
GCAGAGT182150.030.172079
CATGGGG101600.028.1606374
AGAGTAC168200.026.57416210-11
ATGGGGG57050.024.3978355
GTATACG2701.7062121E-924.2520451
AGTACTT86450.024.04577412-13
GAGTACT79650.023.8584212-13
CAGAGTA177700.023.41298910-11
Produced by FastQC (version 0.11.5)