FastQCFastQC Report
Fri 10 Feb 2017
ERR1147790.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147790.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3301348
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183670.5563484976439926No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104330.31602242477921144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83480.25286640487461487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126400.055.7465251
GGTATCA65500.050.063761
AACGCAG159550.043.8769236
TCAACGC166500.042.0811464
CAACGCA168400.041.5710375
ATCAACG170600.041.174413
TATCAAC181500.038.8994752
ACGCAGA185050.037.862237
CGCAGAG189350.036.969318
GCAGAGT206750.033.8867539
GTACATG183000.031.1224651
ACATGGG183700.030.3703783
TACATGG189700.029.9123632
CATGGGG99900.028.5780264
AGAGTAC199700.027.93540610-11
GAGTACT99250.025.64740612-13
ATGGGGG51350.025.250655
CAGAGTA204450.025.1773610-11
AGTACTT108500.025.13273812-13
CATGGGT34500.022.756814
Produced by FastQC (version 0.11.5)