FastQCFastQC Report
Fri 10 Feb 2017
ERR1147789.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147789.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3934233
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218210.5546443233026616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115680.29403444076647217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105880.26912488406253515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA159500.051.3250051
GGTATCA88850.044.828651
AACGCAG190300.042.5756076
TCAACGC205850.039.5039184
TATCAAC208000.039.3534772
ATCAACG209300.038.90963
CAACGCA209600.038.8539055
ACGCAGA218350.037.078487
CGCAGAG220750.036.7250568
GTACATG212250.034.5861631
ACATGGG213100.033.888923
CATGGGG122050.033.4843184
GCAGAGT245350.033.236769
TACATGG221800.032.8282622
ATGGGGG68700.032.2113345
AGAGTAC239250.028.4151910-11
TGGGGGG57100.028.2329126
GAGTACT131800.026.21905312-13
ATGGGAG32100.025.0179235
CATGGGT34150.024.735474
Produced by FastQC (version 0.11.5)