FastQCFastQC Report
Fri 10 Feb 2017
ERR1147788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2528093
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106560.421503481082381No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57840.2287890516685897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52090.2060446352250491No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79600.054.4409751
GGTATCA42300.051.3641471
AACGCAG102250.042.1767276
TCAACGC104350.041.042924
CAACGCA106050.040.553275
ATCAACG106900.040.063883
TATCAAC115250.037.5232352
ACGCAGA118900.036.220547
CGCAGAG122350.035.197118
GTACATG122250.033.7436371
TACATGG126950.032.4250142
ACATGGG127200.031.8462413
GCAGAGT135800.031.8424959
CATGGGG70600.029.5733574
ATGGGGG39150.028.1085345
AGAGTAC127700.027.69066610-11
TATACCG2507.039489E-1026.1728425
CATGGGT27150.024.1002224
CAGAGTA135200.023.97685810-11
AGTACTT69750.023.91999412-13
Produced by FastQC (version 0.11.5)