FastQCFastQC Report
Fri 10 Feb 2017
ERR1147787.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147787.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6677912
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182510.27330399082827084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94570.14161612192553602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82590.12367638267769927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA179500.051.0780531
AACGCAG199550.045.5205466
TCAACGC214500.042.403374
CAACGCA218600.041.662495
ATCAACG221650.041.035523
ACGCAGA222900.040.698657
GGTATCA92550.039.9478341
CGCAGAG227750.039.7503248
TATCAAC249900.036.682852
GCAGAGT261050.034.8392039
GTACATG248950.033.0262761
ACATGGG245250.032.5994643
TACATGG270900.030.2379022
AGAGTAC251650.029.78184110-11
CATGGGG119700.029.171864
GAGTACT125300.025.65798612-13
ATGGGGG55800.024.0931975
CATGGGA100900.023.7593214
CAGAGTA270100.023.58537710-11
CATGGGT57150.023.315894
Produced by FastQC (version 0.11.5)