FastQCFastQC Report
Fri 10 Feb 2017
ERR1147786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477810
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214060.6155022844836262No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111580.32083408811867237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105350.3029205160718958No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA185150.044.9751361
AACGCAG196250.041.8582846
ACGCAGA222450.036.8747637
CGCAGAG226050.036.3122568
TCAACGC227350.036.289334
ATCAACG228550.036.176873
TATCAAC230100.035.9590232
CAACGCA230450.035.8527875
GGTATCA108650.035.1709251
GTACATG210650.035.122721
ACATGGG203650.034.992083
TACATGG219950.033.453622
CATGGGG106750.033.3776974
GCAGAGT248550.033.2404679
CATGGGT37400.031.8094254
ATGGGGG52700.030.9269685
AGAGTAC243750.028.22066310-11
TGGGGGG41100.026.9195966
GAGTACT138600.025.53406312-13
CATGGGA64150.025.0359754
Produced by FastQC (version 0.11.5)