FastQCFastQC Report
Fri 10 Feb 2017
ERR1147785.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147785.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4490646
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179380.3994525509247444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96020.21382224294678315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89210.19865738693274865No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC48140.1072006121168313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA188250.046.1670761
AACGCAG212950.040.4492346
TCAACGC231500.037.028184
GGTATCA108800.036.766721
CAACGCA234800.036.4824335
ATCAACG236050.036.3648453
TATCAAC238150.036.219432
ACGCAGA239950.035.922147
CGCAGAG244050.035.2903448
GTACATG250450.034.8439561
ACATGGG244350.034.7400973
ATGGGGG86800.033.7182275
CATGGGG142250.033.1619644
TACATGG268150.032.3225752
GCAGAGT272750.031.7513949
TGGGGGG65700.031.5089046
CATGGGT35900.028.0034564
AGAGTAC266650.027.2692710-11
ATGGGAG35450.025.6740595
CATGGGA80050.025.6375894
Produced by FastQC (version 0.11.5)