FastQCFastQC Report
Fri 10 Feb 2017
ERR1147784.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147784.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092208
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104490.337913879014607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57480.18588658977662564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49100.15878621360529435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78950.056.1726761
GGTATCA40450.050.1830251
TCAACGC103300.042.609674
AACGCAG104850.042.320146
CAACGCA105600.041.7942665
ATCAACG108000.040.700283
TATCAAC118450.037.511922
ACGCAGA121400.036.403227
CGCAGAG128150.034.529958
GCAGAGT140450.031.6753549
GTACATG132000.031.2972761
ACATGGG132650.030.2672713
TACATGG137500.029.8922822
AGAGTAC131400.028.06324810-11
CATGGGG75300.026.4622364
GAGTACT66250.023.43129212-13
CAGAGTA140850.022.95008510-11
AGTACTT73900.022.77632112-13
ATGGGGG38400.021.5308065
CATGGGT32150.021.0911664
Produced by FastQC (version 0.11.5)