FastQCFastQC Report
Fri 10 Feb 2017
ERR1147783.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147783.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984252
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76570.385888485938278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48630.24507975801460702No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31810.16031229904266192No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28200.063.7479321
GTATCAA58000.060.039371
TCAACGC76100.045.3384784
ATCAACG77000.044.8858073
CAACGCA78650.043.7928735
AACGCAG81300.042.43866
TATCAAC84200.041.330192
ACGCAGA95800.036.2005967
CGCAGAG98950.035.0464068
GCAGAGT108900.032.117399
GTACATG94700.026.9030821
AGAGTAC101300.026.54115310-11
TACATGG97300.026.1670362
GAGTACT47300.026.03154812-13
ACATGGG99950.025.1756763
CAGAGTA106950.024.80531910-11
GTATCGA1300.002056152322.8779669
GTACTTT54900.022.48208214-15
CATGGGG51700.022.3220864
AGTACTT53750.022.299112-13
Produced by FastQC (version 0.11.5)