FastQCFastQC Report
Fri 10 Feb 2017
ERR1147782.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147782.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578019
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140570.5452636307180048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81780.3172203152885995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63400.24592526276959167No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA126700.043.545771
GGTATCA77400.037.525081
AACGCAG151200.036.3055086
TCAACGC158600.034.536564
ATCAACG159900.034.2557753
CAACGCA161550.033.979535
TATCAAC163350.033.6779172
ACGCAGA170750.032.1138847
CGCAGAG172600.031.8379698
GTACATG161000.030.867641
ACATGGG156350.030.5069733
TACATGG165300.029.8985962
GCAGAGT188150.029.4595369
CATGGGG68850.027.3828054
GAGTACT88850.027.074912-13
AGAGTAC180250.025.35562710-11
AGTACTT99400.024.64998212-13
CATGGGT25850.023.4672534
ATGGGAG25900.022.9626965
CATGGGA71750.022.9604554
Produced by FastQC (version 0.11.5)