FastQCFastQC Report
Fri 10 Feb 2017
ERR1147781.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147781.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4830029
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170100.35217179855441866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89010.18428460781498415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75450.15621024221593702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA157850.054.6094171
AACGCAG185900.045.7537656
GGTATCA78550.045.3209041
TCAACGC190450.044.7856034
ATCAACG192900.044.278453
CAACGCA196400.043.4288065
TATCAAC204700.042.075562
ACGCAGA212500.039.94257
CGCAGAG217300.039.0831228
GCAGAGT242500.035.11989
GTACATG231500.031.9899081
ACATGGG231100.031.219873
TACATGG243100.030.3400252
AGAGTAC235600.028.62585810-11
CATGGGG101650.028.4380044
GAGTACT108750.026.058812-13
CATGGGA110650.025.9099084
ATGGGAG50450.024.7587285
CAGAGTA247950.024.3697110-11
AGTACTT136150.022.4744212-13
Produced by FastQC (version 0.11.5)