FastQCFastQC Report
Fri 10 Feb 2017
ERR1147780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4777044
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198970.4165128058272019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108230.2265627027927731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91690.19193878055132002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA152050.057.0452041
GGTATCA74350.053.16591
TCAACGC194250.044.5871624
AACGCAG195550.044.1994936
CAACGCA198000.043.712665
ATCAACG198150.043.7095953
TATCAAC222350.039.22032
ACGCAGA227000.038.0492177
CGCAGAG231250.037.345638
GCAGAGT256400.033.752039
GTACATG240450.031.9134831
ACATGGG241850.030.8433063
TACATGG252150.030.4565932
CATGGGG112350.027.6909644
AGAGTAC245900.027.30745710-11
GAGTACT115300.025.86946712-13
CAGAGTA253550.024.78287910-11
AGTACTT133200.024.17909612-13
CATGGGT51150.023.026564
GTACTTT136150.021.97334514-15
Produced by FastQC (version 0.11.5)