FastQCFastQC Report
Fri 10 Feb 2017
ERR1147779.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147779.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054996
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121610.3980692609744824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.22510667771741766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57140.18703788810198116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.058.931241
GTATCAA90750.058.5813751
TCAACGC115450.045.546764
CAACGCA117800.044.789635
ATCAACG117950.044.5813753
AACGCAG120150.043.864096
TATCAAC130750.040.5354652
ACGCAGA138800.037.927397
CGCAGAG143100.036.869648
GCAGAGT157950.033.5538989
GTACATG144400.032.2811741
TACATGG150300.030.9094432
ACATGGG147850.030.8181513
CATGGGT38400.029.1223074
AGAGTAC151200.026.96749310-11
GAGTACT73150.026.22408512-13
CATGGGG75400.026.1918334
CAGAGTA156950.025.27839310-11
AGTACTT83400.023.71431212-13
ATGGGGG36750.022.1749365
Produced by FastQC (version 0.11.5)