FastQCFastQC Report
Fri 10 Feb 2017
ERR1147778.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147778.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621175
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63310.39051922216910573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.2540749764831064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29360.18110321217635356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31350.049.9249731
GTATCAA54350.048.506931
TCAACGC69500.037.743234
CAACGCA70950.036.8880355
ATCAACG71300.036.7903823
AACGCAG71800.036.5341846
TATCAAC74550.035.505662
ACGCAGA81100.032.2713437
CGCAGAG84250.031.132488
GTACATG82200.029.2488751
ACATGGG82500.028.5513383
GCAGAGT92100.028.5435319
TACATGG84650.028.1072442
GAGTACT41100.025.46923412-13
CATGGGG40300.024.9440464
AGAGTAC86550.024.87634310-11
AGTACTT45200.023.55372812-13
CAGAGTA91200.022.89060810-11
GTACTTT47550.022.13949814-15
CATGGGT16500.021.9902974
Produced by FastQC (version 0.11.5)