FastQCFastQC Report
Fri 10 Feb 2017
ERR1147777.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147777.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2244289
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105070.4681660873443661No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64790.2886883106409201No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49210.21926766116128538No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43400.053.7483181
GTATCAA78950.052.68581
TCAACGC102300.040.4050564
ATCAACG104150.039.687353
CAACGCA104050.039.668335
AACGCAG104200.039.6683046
TATCAAC111950.037.0815542
ACGCAGA121150.034.1674357
CGCAGAG125100.033.0395978
GTACATG119250.031.1382881
GCAGAGT136000.030.4790259
TACATGG123850.029.8690192
ACATGGG122150.029.7978213
CATGGGG64750.026.9125234
AGAGTAC128350.026.41108110-11
GAGTACT60900.025.14589712-13
ATGGGGG34900.024.7097955
CAGAGTA132750.024.52769910-11
AGTACTT63800.024.32915712-13
GTATAGA6400.023.2449471
Produced by FastQC (version 0.11.5)