FastQCFastQC Report
Fri 10 Feb 2017
ERR1147776.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147776.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445752
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102920.7118786624538649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62430.43181679845506005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49310.34106817766809244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16430.1136432804519724No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA93750.044.453521
AACGCAG110800.037.3682946
GGTATCA58400.035.68081
TCAACGC119150.034.799464
ATCAACG119200.034.734963
CAACGCA121400.034.2034955
TATCAAC122650.033.854912
CGTATAC902.3959888E-433.0492253
CGCAGAG126900.032.809168
ACGCAGA126200.032.8082967
GCAGAGT137000.030.4337949
CATGGGG55400.028.7778824
GTACATG113850.028.6044561
TACATGG116600.028.16272
ACATGGG111700.028.0134323
ATGGGGG26550.026.439385
GAGTACT68750.025.52160512-13
AGTACTT72000.025.36089512-13
AGAGTAC134350.025.34524310-11
CAGAGTA136750.023.03017810-11
Produced by FastQC (version 0.11.5)