FastQCFastQC Report
Fri 10 Feb 2017
ERR1147774.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147774.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2460269
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224960.9143715585572146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120650.49039353013837106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101410.4121906994722935No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30480.12388889182443058No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA185150.047.13051
AACGCAG206400.041.814966
TATCAAC230950.037.652562
TCAACGC232700.037.3190354
ATCAACG234250.037.0467073
ACGCAGA233400.037.028727
CAACGCA235700.036.768335
CGCAGAG235100.036.7334258
GGTATCA110200.035.649561
GCAGAGT251400.034.399069
ACATGGG193750.031.0372583
GTACATG201350.030.715371
CATGGGT33200.030.0985164
TACATGG208250.029.6753352
CATGGGG89600.028.9435924
GAGTACT128400.028.4176512-13
AGAGTAC244950.027.4007710-11
ATGGGAG23200.027.1763945
AGTACTT138000.026.6993612-13
CAGAGTA248600.025.2759210-11
Produced by FastQC (version 0.11.5)