FastQCFastQC Report
Fri 10 Feb 2017
ERR1147772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607090
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135940.37686888877183544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75580.2095317832380118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66650.18477498482155977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA100000.055.8305781
GGTATCA51650.047.9393651
AACGCAG126300.043.8897066
TCAACGC126400.043.8549774
CAACGCA130400.042.4641235
ATCAACG131350.042.1573
ACGCAGA145800.038.0604747
TATCAAC149350.037.5152472
CGCAGAG150450.036.8425528
GTACATG155550.034.323511
ACATGGG157450.032.864473
GCAGAGT169650.032.7430429
TACATGG163400.032.6150552
AGAGTAC158800.028.2388410-11
CATGGGG82600.027.7944014
TATACCG3200.026.0212485
CATGGGA72000.025.1951774
CAGAGTA165600.024.26001410-11
GAGTACT82250.024.00646212-13
AGTACTT94000.023.15683712-13
Produced by FastQC (version 0.11.5)