FastQCFastQC Report
Fri 10 Feb 2017
ERR1147771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2958032
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110400.3732211145788822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57830.19550160376899237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54270.18346657507423855No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77950.059.938041
GGTATCA38200.054.064861
TCAACGC104150.044.1443824
ATCAACG106900.042.9531333
CAACGCA106850.042.9175645
AACGCAG108150.042.4016886
TATCAAC120550.038.6328852
ACGCAGA125300.036.5506327
CGCAGAG130200.035.173888
GTACATG134100.034.308341
TACATGG138400.032.962632
ACATGGG139600.032.1248553
GCAGAGT145850.031.521999
AGAGTAC134050.027.86335410-11
CATGGGG67100.027.4785884
CATGGGT36500.025.7465764
AGTACTT70650.025.12885712-13
GAGTACT64000.024.71964512-13
CAGAGTA142550.023.36476310-11
ATGGGGG33800.022.1722165
Produced by FastQC (version 0.11.5)