FastQCFastQC Report
Fri 10 Feb 2017
ERR1147770.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147770.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296516
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124470.5419949175185368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65310.28438730668543133No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63400.276070360493896No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90650.051.6142461
GGTATCA49750.046.5448231
TCAACGC116650.040.0794334
AACGCAG117150.039.8575976
ATCAACG117950.039.587263
CAACGCA118400.039.4368065
TATCAAC124200.037.5967862
ACGCAGA134300.034.8121037
CGCAGAG140300.033.319728
GTACATG137800.031.9666711
TACATGG140900.031.1986732
ACATGGG137700.031.1015723
GCAGAGT151150.031.0853279
CATGGGG70900.029.447244
ATGGGGG34950.027.5709425
AGAGTAC142450.026.616710-11
GAGTACT66850.025.57837512-13
AGTACTT72600.024.45367812-13
CAGAGTA148750.023.57020410-11
GTACTTT79600.021.59341214-15
Produced by FastQC (version 0.11.5)