FastQCFastQC Report
Fri 10 Feb 2017
ERR1147769.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147769.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3020057
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173830.5755851627965962No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99390.32909974877957604No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80110.2652598941013365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115050.060.486631
GGTATCA56500.058.5811421
TCAACGC153250.045.0642664
ATCAACG154600.044.6707573
AACGCAG155000.044.440356
CAACGCA155550.044.4361765
TATCAAC160300.043.3827932
ACGCAGA180100.038.21387
CGCAGAG183650.037.4713948
GCAGAGT201550.034.232039
GTACATG178650.032.9885831
TACATGG180950.032.3827822
ACATGGG182600.031.664023
CATGGGG86150.029.2069134
AGAGTAC192250.027.93647810-11
CATGGGT58100.027.7455754
GAGTACT94050.027.22479212-13
CAGAGTA198150.025.73891810-11
AGTACTT100000.025.72387112-13
ATGGGGG43250.025.1689745
Produced by FastQC (version 0.11.5)