FastQCFastQC Report
Fri 10 Feb 2017
ERR1147768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051792
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87160.8286809559304501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58690.5580000608485328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.3787821166162131No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA16890.16058308106545782No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16190.15392777279157857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13700.13025389050306524No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111850.033.6966171
AACGCAG123950.030.1382856
TATCAAC132750.028.4092712
GGTATCA80400.028.2895371
ATCAACG133000.028.2664223
TCAACGC134400.027.9277214
CATGGGG48100.027.701824
CAACGCA135400.027.6775265
GAGTACT61200.026.62951512-13
ACGCAGA141250.026.3627837
CGCAGAG142600.026.1524338
GTACATG130250.024.6850811
ACATGGG125450.024.6094573
GCAGAGT152450.024.5797359
TACATGG131200.024.3923592
AGTACTT62650.024.3042912-13
GTATAGA2951.8007995E-1024.2202011
CTCTATA3002.237357E-1023.7938862
GTACTTT69700.023.4246814-15
CATGGGC26350.023.026344
Produced by FastQC (version 0.11.5)