FastQCFastQC Report
Fri 10 Feb 2017
ERR1147767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4870914
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154230.31663461929321685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80060.16436340284390158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72510.1488632318287697No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA147850.052.904831
GGTATCA77500.045.625331
AACGCAG176150.043.964016
TCAACGC178850.043.3668254
CAACGCA182000.042.616245
ATCAACG184200.042.2364123
TATCAAC196450.039.784762
ACGCAGA200450.038.6047027
CGCAGAG203150.037.97068
GCAGAGT230500.033.6458139
GTACATG231550.032.495531
ACATGGG227350.032.2318083
CATGGGG122900.031.1191714
TACATGG251800.029.6693022
ATGGGGG68000.028.952635
AGAGTAC221900.027.05633410-11
GAGTACT96250.025.2721712-13
CAGAGTA230800.024.42825310-11
CATGGGT41850.024.019294
TGGGGGG58700.023.4068416
Produced by FastQC (version 0.11.5)