FastQCFastQC Report
Fri 10 Feb 2017
ERR1147766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5457461
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214610.3932414725455666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117770.21579631993705498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102330.18750477557237696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA176500.052.718091
GGTATCA93050.044.4645421
AACGCAG213050.042.971846
TCAACGC221900.041.311624
CAACGCA225350.040.6527635
ATCAACG226450.040.4290163
ACGCAGA242900.037.739327
CGCAGAG248550.036.9731038
TATCAAC251450.036.645672
GTACATG260100.033.3933751
GCAGAGT276700.033.3191389
ACATGGG260450.032.2505573
TACATGG271400.031.8696632
CATGGGG141450.029.2707064
AGAGTAC268000.028.35236710-11
ATGGGGG75400.027.1402595
GAGTACT128900.024.5483312-13
CAGAGTA277200.024.0748110-11
AGTACTT146950.023.86038612-13
CATGGGA105350.023.0948774
Produced by FastQC (version 0.11.5)