FastQCFastQC Report
Fri 10 Feb 2017
ERR1147765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3567880
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129900.364081751628418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71540.2005112279560972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62510.17520208078747043No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96400.060.0188031
GGTATCA46650.053.8466761
TCAACGC125900.045.258164
ATCAACG128450.044.3596883
CAACGCA129400.044.1719135
AACGCAG129700.044.0238846
TATCAAC140600.040.6560862
ACGCAGA150800.037.7846347
CGCAGAG157150.036.2189948
GTACATG172400.033.629451
GCAGAGT173250.033.0934949
TACATGG177100.032.7471582
ACATGGG175100.032.3036423
CATGGGG97450.028.3199984
AGAGTAC162550.027.5150110-11
ATGGGGG52950.024.600015
GAGTACT75700.024.3952112-13
CAGAGTA169700.024.34048710-11
CATGGGT41800.023.6204914
AGTACTT81950.023.00642412-13
Produced by FastQC (version 0.11.5)