FastQCFastQC Report
Fri 10 Feb 2017
ERR1147764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352619
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87710.3728185481797095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.22205040425160213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40170.17074587937953406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.058.177431
GTATCAA69500.057.038891
TCAACGC92250.042.6144264
CAACGCA94750.041.615575
ATCAACG96150.040.824063
AACGCAG96850.040.713226
TATCAAC105000.037.7238242
ACGCAGA113900.034.566537
CGCAGAG117800.033.4207158
GCAGAGT128950.030.6231469
AGAGTAC119900.026.70973610-11
GTACATG117150.026.471411
TACATGG122250.025.4438952
ACATGGG121300.025.3974723
CAGAGTA123450.024.35191710-11
CATGGGG61300.024.0609474
GAGTACT53150.023.60925312-13
AGTACTT56750.022.05917712-13
CATGGGT28600.021.8345784
AGTACAT94000.021.06781412-13
Produced by FastQC (version 0.11.5)