FastQCFastQC Report
Fri 10 Feb 2017
ERR1147763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076337
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125660.6052004082188971No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77940.3753725912508422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61500.29619469286536815No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43750.062.1764071
GTATCAA80450.053.1233981
TCAACGC107450.039.6341674
ATCAACG107650.039.560533
AACGCAG109750.038.8035626
CAACGCA110550.038.522765
TATCAAC118250.036.3663982
ACGCAGA127150.033.4934437
CGCAGAG129250.033.1293378
GCAGAGT141800.030.2811099
GTACATG114850.029.9560361
TACATGG119150.028.8034762
ACATGGG118400.028.3831023
AGAGTAC135800.026.49515210-11
CATGGGG62550.026.0547184
GAGTACT68000.025.97516812-13
AGTACTT71100.025.34450712-13
CAGAGTA140250.025.03962710-11
GTACTTT75800.023.26303114-15
ATGGGTA14450.022.2274195
Produced by FastQC (version 0.11.5)