FastQCFastQC Report
Fri 10 Feb 2017
ERR1147762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427196
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133200.9332985798727014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83620.5859041084756403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66000.4624452422792665No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21550.15099537835027566No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14530.10180802076239003No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115650.038.2935641
GGTATCA78050.035.158191
AACGCAG133300.033.063726
TCAACGC143850.030.5974664
ATCAACG144900.030.3346983
CAACGCA146450.030.0542565
TATCAAC148050.029.8510272
ACGCAGA153800.028.6566567
CGCAGAG155350.028.4090218
GCAGAGT163150.027.2331059
CATGGGT20700.027.0097874
GAGTACT75500.026.50951212-13
AGTACTT77650.026.34999812-13
CATGGGG57450.025.9864734
ACATGGG126400.025.3162173
GTACATG132150.025.1342281
TACATGG133600.024.7986242
GTACTTT86800.023.05839214-15
AGAGTAC158800.022.97881910-11
ATGGGGG20050.022.2495
Produced by FastQC (version 0.11.5)