FastQCFastQC Report
Fri 10 Feb 2017
ERR1147761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862957
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109500.5877752411891418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70460.3782159223213418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.2830983216467154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA94550.042.183891
GGTATCA60450.038.1107941
AACGCAG112750.035.191636
TCAACGC115500.034.662774
ATCAACG117050.034.102113
CAACGCA117850.033.9210975
TATCAAC120200.033.4054832
ACGCAGA130900.030.3121247
CGCAGAG132900.029.8087948
GCAGAGT142500.027.8423679
GTACATG122100.027.6439911
ACATGGG120000.026.9183673
TACATGG125700.026.880142
CATGGGG55650.026.1897344
CATGGGT22200.025.4566214
GAGTACT62000.025.42295612-13
AGTACTT66750.024.32670412-13
CGTATAC1501.6894306E-423.7952443
AGAGTAC134850.023.37743210-11
CAGAGTA139500.022.34235610-11
Produced by FastQC (version 0.11.5)