FastQCFastQC Report
Fri 10 Feb 2017
ERR1147760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388673
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102270.42814566916442726No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62290.26077240375723254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48030.20107398543040425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83800.057.521591
GGTATCA43250.051.185471
TCAACGC108200.044.193154
AACGCAG108850.043.9838836
ATCAACG109400.043.6540343
CAACGCA110800.043.1561285
TATCAAC113400.042.3773272
ACGCAGA127950.037.4173167
CGCAGAG131300.036.4611248
GTACATG140300.033.6784971
GCAGAGT143150.033.5259449
TACATGG143600.032.8024252
CATGGGG82450.031.9550294
ACATGGG143550.031.9431213
ATGGGGG46700.029.418555
TATACCG2502.3646862E-1128.5474535
AGAGTAC137250.026.90723210-11
GAGTACT58200.025.54505312-13
CAGAGTA142950.024.669510-11
GTATAGG7450.023.9637551
Produced by FastQC (version 0.11.5)