FastQCFastQC Report
Fri 10 Feb 2017
ERR1147759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3288464
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123150.374490947749466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73110.2223226406005965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.1718431462226742No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124000.049.8300971
AACGCAG144500.041.9075366
TCAACGC151500.040.20684
ATCAACG153500.039.6829343
CAACGCA155100.039.196865
GGTATCA67500.039.0675351
TATCAAC160050.038.1315162
ACGCAGA165500.036.5534637
CGCAGAG168150.035.974118
GCAGAGT186850.032.532979
ACATGGG169300.031.9739723
GTACATG176100.031.7749271
CATGGGG94300.031.6037224
TACATGG185300.029.885922
ATGGGGG52300.029.0035155
AGAGTAC184650.026.68636110-11
GAGTACT85100.024.98576412-13
CATGGGT31450.024.7778054
TGGGGGG43100.024.5671566
CAGAGTA189350.022.91427410-11
Produced by FastQC (version 0.11.5)