FastQCFastQC Report
Fri 10 Feb 2017
ERR1147758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4246607
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158140.37239141743043325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89350.2104032701872342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75760.17840125069261178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129200.054.826191
GGTATCA66900.049.827091
AACGCAG161350.043.3561176
TCAACGC162450.043.2090154
CAACGCA165000.042.5051845
ATCAACG167150.041.9940453
TATCAAC184100.038.321542
ACGCAGA185700.037.6705867
CGCAGAG190800.036.7229928
GTACATG201000.033.7311631
GCAGAGT214250.032.7313659
ACATGGG205550.032.0652283
TACATGG213650.031.6569882
CATGGGG102650.029.0908764
AGAGTAC203700.027.1405910-11
ATGGGGG52000.026.7685325
CATGGGT47900.025.9551074
GAGTACT95250.024.977912-13
CAGAGTA210850.024.3584510-11
AGTACTT111700.022.07152212-13
Produced by FastQC (version 0.11.5)