FastQCFastQC Report
Fri 10 Feb 2017
ERR1147757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247447
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90290.4017447352484842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.22509985774970442No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42260.18803557992691264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73900.050.8233071
GGTATCA41350.047.0706561
TCAACGC93300.039.2732434
AACGCAG94500.038.7745326
ATCAACG94400.038.7525983
CAACGCA95600.038.3906025
TATCAAC103100.036.002552
ACGCAGA110500.033.106297
CGCAGAG113200.032.3677568
GTACATG115850.031.7005711
ACATGGG115450.031.4807383
TACATGG119050.030.9291932
TATCACG1602.6105954E-729.7424892
GCAGAGT126800.029.1306849
CATGGGG61100.027.8434144
AGAGTAC118350.025.9334210-11
ATGGGGG33850.025.6561725
GAGTACT53250.024.79771612-13
GTATTAT7400.023.3262161
CAGAGTA123350.022.8569110-11
Produced by FastQC (version 0.11.5)