FastQCFastQC Report
Fri 10 Feb 2017
ERR1147756.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147756.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1644557
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.3943919243905806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41760.25392856556507315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.1880141582201164No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.062.2131731
GTATCAA48100.056.306021
TCAACGC63300.042.193014
CAACGCA64800.041.216325
ATCAACG65200.040.9634553
AACGCAG65750.040.711276
TATCAAC73000.036.8298722
ACGCAGA79500.033.6689877
CGCAGAG81000.032.897628
GTACATG82750.031.7218931
TACATGG85650.030.3486062
GCAGAGT89800.029.9387449
ACATGGG85450.029.6548293
TATAACG1254.894023E-528.5513022
CATGGGT21500.027.3901634
GAGTACT38650.026.23814812-13
ATGGGGG22700.026.2042685
CATGGGG46600.025.7847754
AGAGTAC83350.025.68947210-11
CAGAGTA86750.024.61405810-11
Produced by FastQC (version 0.11.5)