FastQCFastQC Report
Fri 10 Feb 2017
ERR1147754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071689
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100520.4852079631643553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68630.3312755920410834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50180.24221782323505123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85550.045.227421
GGTATCA51550.044.226091
TCAACGC105800.035.9286234
AACGCAG106800.035.536516
CAACGCA107250.035.331945
ATCAACG108150.035.202933
TATCAAC111650.034.3674472
ACGCAGA122750.030.9189347
CGCAGAG123500.030.8245288
GCAGAGT137350.027.802889
GAGTACT58800.026.55433312-13
GTACATG111500.025.0383931
ACATGGG107500.025.0123963
TACATGG114450.023.9624632
AGAGTAC127950.023.6857610-11
CAGAGTA132400.023.06937810-11
GTACTTT68250.022.92115614-15
AGTACTT64050.022.84543212-13
CATGGGG52000.022.0789954
CATGGGT21400.021.1263834
Produced by FastQC (version 0.11.5)