FastQCFastQC Report
Fri 10 Feb 2017
ERR1147753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872941
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111310.3874426937413612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70780.2463677465008853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.18622032265890598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.059.0314061
GTATCAA88850.054.6710971
TCAACGC117800.041.0018124
CAACGCA120900.039.9996835
AACGCAG122400.039.558096
ATCAACG122650.039.3319633
TATCAAC133150.036.765752
ACGCAGA143600.033.635197
CGCAGAG148050.032.623078
GCAGAGT160200.030.2602379
GTACATG147600.028.5139981
TACATGG155450.027.091142
ACATGGG153300.026.7731383
AGAGTAC152200.025.69512610-11
CATGGGG83150.024.8233034
GAGTACT65350.024.5291712-13
CAGAGTA157050.024.5228810-11
ATGGGGG40500.022.9119425
GTACTTT72700.022.0492614-15
AGTACTT70550.021.24580412-13
Produced by FastQC (version 0.11.5)