FastQCFastQC Report
Fri 10 Feb 2017
ERR1147752.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147752.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272767
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85700.37707340875681494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57760.2541395576405325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.18796471437679269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.061.333291
GTATCAA65950.054.7858281
TCAACGC86300.041.5646064
CAACGCA88300.040.5557985
ATCAACG90250.039.7454343
AACGCAG90200.039.7015156
TATCAAC96850.037.1597562
ACGCAGA105050.034.088517
CGCAGAG109750.032.6785668
GTACATG112300.031.3257941
TACATGG114150.030.5899872
ACATGGG114850.029.9373913
GCAGAGT120950.029.7016989
AGAGTAC112150.026.5692410-11
CATGGGG60900.026.5686054
CAGAGTA115950.024.57001710-11
GAGTACT49750.024.14899312-13
AGTACTT53500.023.56800312-13
ATGGGGG31500.022.2837665
CATGGGT23550.022.2284364
Produced by FastQC (version 0.11.5)